Abstract: In this talk, we will explore the intriguing interactions between DNA and gold nanoparticles, focusing on their newly explored binding mechanisms. Key topics will include the selective binding of gold nanoparticles to mismatched and/or single-stranded regions of double-stranded DNA and the subsequent movement of these particles along the DNA molecule over the long molecular distances. Additionally, we will explore the interaction between silver ions and double-stranded DNA, composed of guanine and cytosine nucleotides, leading to the formation of extended silver ion chains within the DNA core. Shifting from molecular interactions to material applications, we will discuss the assembly of gold nanoparticles on silicon and mica surfaces, resulting in the formation of well-ordered nanoparticle layers. Finally, we will also touch on important recent biomedical applications of such small nanoparticle demonstrating significant inhibition of tumor growth in mice via illumination. By integrating together these diverse topics, this presentation highlights the transformative potential of nanoparticles in advancing DNA research, materials science, and cancer treatment strategies.

Bio: Alexander B. Kotlyar is a Professor of Biochemistry at Tel Aviv University. Over the past decade, his research has primarily focused on the synthesis and characterization of novel DNAbased nanostructures. Prof. Kotlyar's laboratory explores multiple directions within this field, including: 1 – Enzymatic synthesis of long (kilobase-length) DNA-based molecular structures capable of conducting electrical currents, such as G4-DNA (G-quadruplex), GC base pair DNA-silver complexes, E-DNA, and DNA-gold nanoparticle complexes with metallic conductivity; 2 - Targeted cancer therapies using gold nanoparticles and nanorods coated with proteins that selectively bind to receptors overexpressed in cancer cells; 3 - Investigating mitochondrial inhibition by derivatives of nicotinamide dinucleotide, particularly targeting Complex I of the respiratory chain.