Publications

(89) Torrey, Z. R.; Halbers, L. P.; Scipioni, L.; Tedeschi, G.; Digman, M. A.; .; Prescher, J. A. A versatile bioluminescent probe with tunable color. RSC Chem. Biol. 2024, 5, 1097-1103.

(88) Tedeschi, G; Navarro, M. X.; Scipioni, L; Sondhi, T. K.; Prescher J. A.*; Digman, M. A.* Monitoring macrophage polarization with gene expression reporters and bioluminescence phasor analysis.. ACS Chem. Biomed. Imaging 2024. In Press

(87) Pratt, M. R.; Prescher, J. A. Carolyn Bertozzi: Building new bonds between molecules, fields, and communities.. Cell Chem. Biol. 2024, 31, 1383-1385.

(86) Labra, B.; Parag-Sharma, K.; Powers, J. J.; Walker, J. R.; Kirkland, T. A.; Brennan, C. K.; Prescher, J. A.; Amelio, A. L. Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporter . iScience 2024, 27, 110655.

(85) Townsend, Kat M.; Prescher, J. A. Recent advances in bioluminescent probes for neurobiology. Neurophotonics 2024, 11, 24204.

(84) Caldwell, Donald R.; Townsend, Katherine M.; Kolbab-Kartchner, Bethany; Hadjian, Tanya; Ivanic, Joseph; Love, Anna C.; Malvar, Beatrice; Mills, Jeremy; Prescher, Jennifer A.; Schnermann, Martin J. Expedient synthesis and characterization of pi-extended luciferins. J. Org. Chem. 2024, 89, 14625-14633.

(83) Navarro, M. X.; Brennan, C. K.; Love, A. C.; Prescher, J. A. Caged luciferins enable rapid multicomponent bioluminescence imaging. Photochem. Photobiol. 2024, 100, 67-74.

(82) Li, Yong; Townsend, Katherine M.; Dorn, Robert S.; Prescher, Jennifer A.; Potma, Eric O. Enhancing alkyne-based raman tags with a sulfur linker. J. Phys. Chem. B. 2023, 127, 1976-1982.

(81) Love, A. C.; Caldwell, D. R.; Kolbaba-Kartchner, B.; Townsend, K. M.; Halbers, L. P.; Yao, Z.; Brennan, C. K.; Ivanic, J.; Hadijian, T.; Mills, J. H.; Schnermann, M. J.; Prescher, J. A. Red-shifted coumarin luciferins for improved bioluminescence imaging. J. Am. Chem. Soc. 2023, 145, 3335-3345.

(80) Williams, S. J.; Gewing-Mullins, J. A.; Lieberman, W. K.; Kolbaba-Kartchner, B.; Iqbal, R.; Burgess, H. M.; Colee, C. M.; Ornelas, M. Y.; Reid-McLaughlin, E. S.; Mills, J. H.; Prescher, J. A.; Leconte, A. M. Biochemical analysis leads to improved orthogonal bioluminescent tools. ChemBioChem 2023, 24, e202200726.

(79) Ng, Kevin.; Prescher, J. A. Generalized bioluminescent platform to observe and track cellular interactions. Bioconjug. Chem. 2022, 33, 1876-1884.

(78) Dorn, R. S.; Prescher, J. A. Bioorthogonal phosphines: then and now. Isr. J. Chem. 2022, 63, e202200070.

(77) Brennan, C. K.; Yao, Z. W.; Ionkina, A. A.; Rathbun, C. M.; Sathishkumar, B.; Prescher, J. A. Multiplexed bioluminescence imaging with a substrate unmixing platform. Cell Chem. Biol. 2022, 29, 1649-1660.

(76) Ramirez-Corona, B. A.*; Love, A. C.*; Chandrasekaran, S.; Prescher, J. A.; Wunderlich, Z. Longitudinal monitoring of individual infection progression in Drosophila melanogaster. iScience 2022, 25,. (Originally uploaded to BioRxiv: https://www.biorxiv.org/content/10.1101/2021.08.17.456698v1)

(75) Li, Enya; Brennan, C. K.; Ramirez, A.; Tucker, J. A.; Butkovich, N.; Meli, V. S.; Ionkina, A. A.; Nelson, E. L.; Prescher, J. A.; Wang, S.-W. Macromolecular assembly of bioluminescent protein nanoparticles for enhanced imaging. Materials Today Bio. 2022, 17, 100455.

(74) Ng, K. K.; Cole, K. H.; Halbers, L. P.; Chen, C.; Barhoosh, A. A.; Reid-McLaughlin, E.; Metcalfe, M.; Kent, A. D.; Steward, O.; Luptak, A.; Prescher, J. A. A modular platform for bioluminescent RNA tracking. BioRxiv 2022. Preprint

(73) Yao, Z.*; Brennan, C. K.*; Scipioni, L.*; Chen, H.; Ng, K.; Digman, M. A.; Prescher, J. A. Multicomponent bioluminescence microscopy via phasor analysis. Nat. Methods 2022, 19, 893-898.

(72) Heiss, T. K.; Dorn, R. S.; Ferreira, A. J.; Love, A. C.; Prescher, J. A. Fluorogenic Cyclopropenones for Multicomponent, Real-Time Imaging. J. Am. Chem. Soc. 2022, 144, 7871-7880.

(71) Ng, K. K.; Reinert, Z. E.; Corver, J.; Resurreccion, D.; Hensbergen, P. J.; Prescher, J. A. A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker. Sensors 2021, 21, 7485-7495.

(70) Brennan, C. K.; Ornelas, M. Y.; Yao, Z. W.; Prescher, J. A. Multicomponent Bioluminescence Imaging with Naphthylamino Luciferins. ChemBioChem 2021, 22, 2650-2654.

(69) Love, A. C.; Tran, S. H.; Prescher, J. A. Caged Cumate Enables Proximity-Dependent Control Over Gene Expression. ChemBioChem 2021, 22, 2440-2448.

(68) Heiss, T. K.*; Dorn, R. S.*; Prescher, J. A. Bioorthogonal reactions of triarylphosphines and related analogs. Chem. Rev. 2021, 121, 6802-6849. (Invited Article)

(67) Yao, Z.*; Caldwell, D.*; Love, A. C.*; Kolbaba-Kartchner, B.; Mills, J.; Schnermann, M.; Prescher, J. A. Coumarin Luciferins and Mutant Luciferases for Robust Multi-component Bioluminescence Imaging. Chem. Sci. 2021, 12, 11684-11691. (Originally uploaded to ChemRxiv: https://doi.org/10.26434/chemrxiv.14544630.v1)

(66) Ionkina, A. A.; Balderrama-Gutierrez, G.; Ibanez, K. J.; Phan, S. H. D.; Cortez, A. N.; Mortazavi, A.; Prescher, J. A. Transcriptome analysis of heterogeneity in mouse model of metastatic breast cancer. Breast Cancer Res. 2021, 23, 93. (Originally uploaded to biorxiv: https://doi.org/10.1101/2021.05.04.442511)

(65) Scinto, S. L.; Bilodeau, D. A.; Hicapie, R.; Lee, W.; Nguyen, S. S.; Xu, M.; am Ende, C. W.; Finn, M. G.; Lang, K.; Lin, Q.; Pezacki, J. P.; Prescher, J. A.; Robillard, M. S.; Fox, J. M. Bioorthogonal chemistry. Nat. Rev. Methods Primers 2021, 1, Article 30.

(64) Williams, S. J.; Hwang, C. S.; Prescher, J. A. Orthogonal Bioluminescent Probes from Disubstituted Luciferins. Biochemistry 2021, 60, 563-572.

(63) Unger, E. K.; Keller, J. P.; Altermatt, M.; Liang, R.; Matsui, A.; Dong, C.; Hon, O. J.; Yao, Z.; Sun, J.; Banala, S.; Flanigan, M. E.; Jaffe, D. A.; Hartanto, S.; Carlen, J.; Mizuno, G. O.; Borden, P. M.; Shivange, A. V.; Cameron, L. P.; Sinning, S.; Underhill, S. M.; Olson, D. E.; Amara, S. G.; Lang, D. T.; Rudnick, G.; Marvin, J. S.; Lavis, L. D.; Lester, H. A.; Alvarez, V. A.; Fisher, A. J.; Prescher, J. A.; Kash, T. L.; Yarov-Yarovoy, V.; Gradinaru, V.; Looger, L. L.; Tian, L. Directed Evolution of a Selective and Sensitive Serotonin Sensor via Machine Learning. Cell 2020, 183, 1986-2002.e26.

(62) Row, R. D.; Nguyen, S. S.; Ferreira, A. J.; Prescher, J. A. Chemically triggered crosslinking with bioorthogonal cyclopropenones. Chem. Commun. 2020, 56, 10883-10886.

(61) Yao, Z.; Zhang, B. S.; Steinhardt, R. C.; Mills, J. H.; Prescher, J. A. Multicomponent Biolumiescence Imaging with a pi-Extended Luciferin. J. Am. Chem. Soc. 2020, 142, 14080-14089.

(60) Love, A. C.; Prescher, J. A. Seeing (And Using) the Light: Recent Developments in Bioluminescence Technology. Cell Chem. Biol. 2020, 27, 904-920.

(59) Nguyen, S. S.; Prescher, J. A. Developing bioorthogonal probes to span a spectrum of reactivities. Nat. Rev. Chem. 2020, 4, 476-489.

(58) Rathbun, C. M.*; Ionkina, A. I.*; Yao, Z.*; Jones, K. A.; Porterfield, W. B.; Prescher, J. A. Rapid Multicomponent Bioluminescence Imaging via Substrate Unmixing. ACS Chem. Biol. 2021, 16, 682-690. (Originally uploaded to biorxiv: https://doi.org/10.1101/811026)

(57) Nguyen, S. S.*; Ferreira, A. J.*; Long, Z. G.; Heiss, T. K.; Dorn, R. S.; Row, R. D.; Prescher, J. A. Butenolide synthesis from functionalized cyclopropenones. Org. Lett. 2019, 21, 8695-8699.

(56) Williams, S. J.; Prescher, J. A. Building Biological Flashlights: Orthogonal Luciferases and Luciferins for in Vivo Imaging. Acc. Chem. Res. 2019, 52, 3039-3050.

(55) Kamber, D. N.*; Nguyen, S. S.*; Liu, F.; Briggs, J. S.; Shih, H.-W.; Row, R. D.; Long, Z. G.; Houk, K. N.; Liang, Y.; Prescher, J. A. Isomeric triazines exhibit unique profiles of bioorthogonal reactivity. Chem. Sci. 2019, 10, 9109-9114.

(54) Heiss, T. K.; Prescher, J. A. Cyclopropeniminium ions exhibit unique reactivity profiles with bioorthogonal phosphines. J. Org. Chem. 2019, 84, 7443-7448.

(53) Row, R. D.; Prescher, J. A. A Cyclopropenethione-Phosphine Ligation for Rapid Biomolecule Labeling. Org. Lett. 2018, 20, 5614-5617.

(52) Yao, Z.*; Zhang, B. S.*; Prescher, J. A. Advances in bioluminescence imaging: new probes from old recipes. Curr. Opin. Chem. Biol. 2018, 45, 148-156.

(51) Row, R. D.; Prescher, J. A. Constructing New Bioorthogonal Reagents and Reactions. Acc. Chem. Res. 2018, 51, 1073-1081.

(50) Yu, T.; Laird, J. R.; Prescher, J. A.; Thorpe, C. Gaussia princeps luciferase: A bioluminescent substrate for oxidative protein folding. Protein Sci. 2018, 27, 1509-1517.

(49) Zhang, B. S.; Jones, K. A.; McCutcheon, D. C.; Prescher, J. A. Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging. ChemBioChem 2018, 19, 470-477.

(48) Heemstra, J. M.; Waterman, R.; Antos, J. M.; Beuning, P. J.; Bur, S. K.; Columbus, L.; Feig, A. L.; Fuller, A. A.; Gillmore, J. G.; Leconte, A. M.; Londergan, C. H.; Pomerantz, W. C. K.; Prescher, J. A.; Stanley, L. M. Throwing Away the Cookbook: Implementing Course-Based Undergraduate Research Experiences (CUREs) in Chemistry. ACS Symposium Series 2017, 1248, 33-63. (Chapter 3 of the "Educational and Outreach Projects from the Cottrell Scholars Collaborative Undergraduate and Graduate Education, Volume 1")

(47) Liu, M. D.; Warner, E. A.; Morrissey, C. E.; Fick, C. W.; Wu, T. S.; Ornelas, M. Y.; Ochoa, G. V.; Zhang, B.; Rathbun, C. M.; Porterfield, W. B.; Prescher, J. A.; Leconte, A. M. Statistical Coupling Analysis-guided library design for discovery of mutant luciferases. Biochemistry 2017, 57, 663-671.

(46) Rathbun, C. M.*; Porterfield, W. B.*; Jones, K. A.*; Sagoe, M. J.; Reyes, M. R.; Hua, C. T.; Prescher, J. A. Parallel Screening for Rapid Identification of Orthogonal Bioluminescent Tools. ACS Cent. Sci. 2017, 3, 1254-1261.

(45) Rathbun, C. M.; Prescher, J. A. Bioluminescent Probes for Imaging Biology beyond the Culture Dish. Biochemistry 2017, 56, 5178-5184. (invited article for "Seeing Into Cells" issue)

(44) Gartner, Z. J.; Prescher, J. A.; Lavis L. D. Unraveling cell-to-cell signaling networks with chemical biology. Nat. Chem. Biol. 2017, 13, 564-568.

(43) Row, R. D.; Shih, H.-W.; Alexander, A. T.; Mehl, R. A.; Prescher, J. A. Cyclopropenones for metabolic targeting and sequential bioorthogonal labeling. J. Am. Chem. Soc. 2017, 139, 7370-7375.

(42) Jones, K. A.*; Porterfield, W. B.*; Rathbun, C. M.*; McCutcheon, D. C.; Paley, M. A.; Prescher, J. A. Orthogonal luciferase-luciferin pairs for bioluminescence imaging. J. Am. Chem. Soc. 2017, 139, 2351-2358.

(41) Row, R. D.; Prescher, J. A. Tetrazine Marks the Spot. ACS Cent. Sci. 2016, 2, 493-494.

(40) Steinhardt, R. C.; Rathbun, C. M.; Krull, B. T.; Yu, j.; Yang, Y.; Nguyen, B. D.; Kwon, J.; McCutcheon, D. C.; Jones, K. A.; Furche, F.; Prescher, J. A. Brominated luciferins are versatile bioluminescent probes. ChemBioChem 2016, 18, 96-100.

(39) Steinhardt, R. C.; O'Neill, J. M.; Rathbun, C. M.; McCutcheon, D. C.; Paley, M. A.; Prescher, J. A. Design and synthesis of an alkynyl luciferin analog for bioluminescence imaging. Chem. Eur. J. 2016, 22, 3671-3675.

(38) Nazarova, L. A.; Ochoa, R. A.; Jones, K. A.; Morrissette, N. M.; Prescher, J. A. Extracellular Toxoplasma gondii tachyzoites metabolize and incorporate unnatural sugars into cellular proteins. Microbes Infect. 2015, 18, 199-210.

(37) Shih, H.-W.; Prescher, J. A. A bioorthogonal cyclopropenenones ligation mediated by triarylphosphines. J. Am. Chem. Soc. 2015, 137, 10036-10039.

(36) Patterson, D. M.; Prescher, J. A. Orthogonal bioorthogonal chemistries. Curr. Opin. Chem. Biol. 2015, 28, 141-149.

(35) Porterfield, W. B.; Jones, K. A.; McCutcheon, D. C.; Prescher, J. A. A "caged" luciferin for imaging cell-cell contacts. J. Am. Chem. Soc. 2015, 137, 8656-8659.

(34) Kamber, D. N.; Liang, Y.; Lopez, S. A.; Houk, K. N.; Mehl, R. A.; Prescher, J. A. 1,2,4-Triazines are versatile bioorthogonal reagents. J. Am. Chem. Soc. 2015, 137, 8388-8391.

(33) Jones, K. A.; Li, D. J.; Hui, E. E.; Sellmyer, M. A.; Prescher J. A. Visualizing cell proximity with genetically encoded bioluminescent reporters. ACS Chem. Biol. 2015, 10, 933-938.

(32) McCutcheon, D. C.; Porterfield, W. B.; Prescher, J. A. Rapid and scalable assembly of firefly luciferase substrates. Org. Biomol. Chem. 2015, 13, 2117-2121.

(31) Porterfield, W. B.; Prescher, J. A. Tools for visualizing cell-cell interactomes. Curr. Opin. Chem. Biol. 2015, 24, 121-130. (invited article for "Omics" issue)

(30) Smith, B. R.; Ghosn, E. E.; Rallapalli, H.; Prescher, J. A.; Larson, T.; Herzenberg L. A.; Gambhir, S. S. Selective uptake of single-walled carbon nanotubes by circulating monocytes for enhanced tumour delivery. Nat. Nanotechnol. 2014, 9, 481-487.

(29) Shih, H.-W.; Kamber, D. N.; Prescher, J. A. Building better bioorthogonal reactions. Curr. Opin. Chem. Biol. 2014, 21, 103-111. (invited article for "In Vivo Chemistry" issue)

(28) Patterson, D. M.; Jones, K. A.; Prescher, J. A. Improved cyclopropene reporters for probing protein glycosylation. Mol. BioSyst. 2014, 10, 1693-1697. (invited article for "Emerging Investigators" issue)

(27) Patterson, D. M.*; Nazarova, L. A.*; Prescher, J. A. Finding the right (bioorthogonal) chemistry. ACS Chem. Biol. 2014, 9, 592-605.

(26) Evans, M. S.; Adams, S. T.; Reddy, G. R.; Paley, M. A.; Aronin, N.; Prescher, J. A.*; Miller S. C.* A synthetic luciferin improves bioluminescence imaging in live mice. Nat. Methods 2014, 11, 393-395.

(25) Paley, M. A.; Prescher, J. A. Bioluminescence: A versatile technique for imaging cellular and molecular features.. MedChemComm 2014, 5, 255-267.

(24) Kamber, D. N.; Nazarova, L. A.; Liang, Y.; Lopez, S. A.; Patterson, D. M.; Shih, H.-W.; Houk, K. N.; Prescher, J. A. Isomeric cyclopropenes exhibit unique bioorthogonal reactivities. J. Am. Chem. Soc. 2013, 135, 13680-13683.

(23) Sellmyer, M. A.; Bronsart, L.; Imoto, H.; Contag, C. H.; Wandless, T. J.; Prescher, J. A. Visualizing cellular interactions with a generalized proximity reporter. Proc. Natl. Acad. Sci. U.S.A. 2013, 110, 8567-8572.

(22) Patterson, D. M.; Nazarova, L. A.; Xie, B.; Kamber, D. N.; Prescher, J. A. Functionalized cyclopropenes as bioorthogonal chemical reporters. J. Am. Chem. Soc. 2012, 134, 18638-18643.

(21) McCutcheon, D. C.; Paley, M. A.; Steinhardt, R. C.; Prescher, J. A. Expedient synthesis of electronically modified luciferins for bioluminescence imaging. J. Am. Chem. Soc. 2012, 134, 7604-7607.

(20) Prescher, J. A.; Contag, C. H. Imaging mouse models of human cancer. Genetically Engineered Mice for Cancer Research 2011. Eds (Springer): Green, J. and Ried, T.